To calibrate, remove all brightly colored red, blue, and green objects from the frame. Calibrate the sliders so no centroids appear (i.e., centroids are (NaN, NaN) and canvas is entirely gray). Then, insert one marker at a time, calibrating the sliders as necessary, making sure only the markers appear in the canvas. If done correctly, the centroids should update to the center of the marker. This can be confirmed by a white dot located in the center of the colored region.

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